Rabbit anti-CD4 Recombinant Monoclonal Antibody [BLR167J]

• Catalog Number: orb1519574
• Category: Antibodies
• Description: CD4 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: IHC, IP, WB
• Reactivity: Mouse
• Immunogen: Between 407 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD4
• UniProt ID: P06332
• NCBI: NP_038516.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: L3T4; T-cell surface glycoprotein CD4; T-cell surf
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse CD4 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, BW5147.3, thymus, 70Z/3, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD4 by immunohistochemistry. Sample: FFPE section of mouse thymus. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD4 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (200 µg per IP reaction; 20% of IP loaded) from mouse thymus. Antibodies: Rabbit anti-CD4 recombinant monoclonal antibody (orb1519574) used for IP at 6 µl/200 µg lysate. CD4 was also immunoprecipitated by a previous lot of this antibody (orb1519574). For blotting immunoprecipitated CD4, orb1519574 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.