Rabbit anti-c-Kit Recombinant Monoclonal Antibody

• Catalog Number: orb1733237
• Category: Antibodies
• Description: c-Kit Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR263L
• Tested applications: ICC, IHC, WB
• Reactivity: Human
• Immunogen: Between 325 and 375
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: c-Kit
• UniProt ID: P10721
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: piebald trait protein; proto-oncogene c-Kit; v-kit
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human c-KIT by western blot. Samples: Whole cell lysate (50 µg) from GaMG, HEL 92.1.7, HEK293T, HUVEC-C, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733237) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human c-KIT by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733237). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human c-KIT by immunocytochemistry. Sample: FFPE section of human HEL 92.1.7 cells. Antibody: Rabbit anti-c-KIT recombinant monoclonal antibody (orb1733237). Secondary: HRP-conjugated goat anti-rabbit IgG.