Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody

• Catalog Number: orb1806460
• Category: Antibodies
• Description: Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR279L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 750 and 800
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: WB: 1:1000 (3-8% Tris-acetate gel recommended), IP: 20 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ARID1A/BAF250
• UniProt ID: O14497
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: osa homolog 1; SWI/SNF-related, matrix-associated,
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, RKO, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1A/BAF250 by western blot. Samples: Whole cell lysate (50 µg) from F9, NIH 3T3, A20, CH27, and ND7/23 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1A/BAF250 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti- ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1A/BAF250 recombinant monoclonal antibody (orb1806460) used for IP at 20 µl/mg lysate.

Detection of mouse ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of mouse mIMCD-3 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1A/BAF250 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-ARID1A recombinant monoclonal antibody (orb1806460). Secondary: HRP-conjugated goat anti-rabbit IgG.