Rabbit anti-alpha-Tubulin Antibody, Affinity Purified

• Catalog Number: orb1520420
• Category: Antibodies
• Description: alpha-Tubulin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 175 and 225
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2-10 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: alpha-Tubulin
• UniProt ID: Q71U36
• NCBI: NP_001257328.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: tubulin alpha-1A chain; hum-a-tub1; tubulin, alpha
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse TUBA1A by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TUBA1A antibody (orb1520420) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TUBA1A by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TUBA1A antibody (orb1520420) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human TUBA1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TUBA1A antibody (orb1520420) used for IP at 1 µg per reaction.