Rabbit anti-Alpha-Adducin Antibody, Affinity Purified

• Catalog Number: orb1524322
• Category: Antibodies
• Description: Alpha-Adducin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 650 and 700
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Alpha-Adducin
• UniProt ID: P35611
• NCBI: NP_001110.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ADDA; Erythrocyte adducin subunit alpha; erythrocy
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Alpha-Adducin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Alpha-Adducin antibody orb1524322 used for IP at 6 µg per reaction.

Detection of human Alpha-Adducin by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Alpha-Adducin (Cat. No. orb1524322) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Alpha-Adducin by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Alpha-Adducin Cat. No. orb1524322 used at a dilution of 1: 5, 000 (0.2µg/ml) (right) and Cat. No. orb1524325 used at a dilution of 1: 5, 000 (0.2µg/ml) (left). Detection: DAB

Detection of human Alpha-Adducin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Alpha-Adducin antibody orb1524322 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.