Rabbit anti-AKT1 Recombinant Monoclonal Antibody

• Catalog Number: orb1733248
• Category: Antibodies
• Description: AKT1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR245L
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 100 and 150
• Antibody Type: Primary Antibody
• Concentration: 400 µg/ml
• Dilution range: WB: 1:1000
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: AKT1
• UniProt ID: P31749
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Protein kinase B; RAC; v-akt murine thymoma viral
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse AKT1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733248) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human AKT1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, A-549, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733248) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse AKT1 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733248) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human AKT1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733248) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.