RAB1A antibody

• Catalog Number: orb667868
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RAB1A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human RAB1A. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RAB1A
• Entrez: 19324, 5861, 81754
• UniProt ID: P62821, P62820, Q6NYB7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-RAB1 antibody, anti-Ras-related protein Rab-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAB1A expression in U87MG (A), mouse brain (B), rat testis (C) whole cell lysates. (Predicted band size: 13; 15; 22 kD; Observed band size: 23 kD)

Immunofluorescent analysis of RAB1A staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).