Pyruvate Kinase (PKM2) Antibody (C-term)

• Catalog Number: orb527246
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKM
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3697
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:2000, IF: 1:200, IHC-P: 1:50- 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937
• Target: PKM
• UniProt ID: P14618
• NCBI: NP_872271.1, NP_001193727.1, NP_872270.1, NP_001193726.1, NP_001193725.1, NP_001193728.1, NP_002645.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-hPKM2-N491 at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: C2C12 whole cell lysate. Lane 5: PC-12 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PKM2 Antibody (N491) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) (C-term) Antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Pyruvate Kinase (PKM2) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Pyruvate Kinase (PKM2) Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of hPKM2-N491 in A2058 cell line (lane 1) and mouse brain tissue (lane 2) lysates (35 ug/lane). PKM2 (arrow) was detected using the purified Pab.