Pyruvate Kinase (PKM2) Antibody (N-term)

• Catalog Number: orb1939500
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5870
• Tested applications: WB
• Predicted Reactivity: Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937 Da
• Target: This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-151 amino acids from the N-terminal region of human Pyruvate Kinase (PKM2).
• UniProt ID: P14618
• NCBI: NP_001193728.1, NP_001193725.1, NP_002645.3, NP_872270.1, NP_872271.1, NP_001193727.1, NP_001193726.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PKM2 (K136) antibody at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: NIH/3T3 whole cell lysates. Lane 4: PC-12 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PKM2 (K136) antibody at 1:2000 dilution. Lane 1: MCF-7 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: THP-1 whole cell lysates. Lane 4: NIH/3T3 whole cell lysates. Lane 5: PC-12 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.