PU.1 antibody

• Catalog Number: orb1151709
• Category: Antibodies
• Description: Mouse monoclonal antibody to PU.1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PU1/2118
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: A portion of amino acids 16-170 was used as the immunogen for the PU.1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PU.1 antibody is available for research use only.
• UniProt ID: P17947
• Storage: Aliquot the PU.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PU.1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon carcinoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hodgkins Lymphoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Lymph Node tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2118) as confirmation of integrity and purity.