PTTG1 Antibody

• Catalog Number: orb1263285
• Category: Antibodies
• Description: PTTG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PTTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human PTTG1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:500For IF starting dilution is: 1:100For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 22 kDa
• Target: PTTG1
• UniProt ID: O95997
• NCBI: O95997
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Securin, Esp1-associated protein, Pituitary tumor-
• Note: For research use only
• Application notes: For WB starting dilution is: 1:500For IF starting dilution is: 1:100For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.

Immunofluorescent analysis of U251 cells, using PTTG1 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Fluor 554 (red) conjugated Phalloidin (red).

Formalin-fixed and paraffin-embedded human testis tissue with PTTG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.