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Catalog Number | orb1264586 |
---|---|
Category | Antibodies |
Description | PTPN6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This PTPN6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-277 amino acids from the Central region of human PTPN6. |
Concentration | batch dependent |
Dilution range | For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 68 kDa |
Target | PTPN6 |
UniProt ID | P29350 |
NCBI | P29350 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Tyrosine-protein phosphatase non-receptor type 6, Read more... |
Note | For research use only |
Application notes | For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of U251 cells, using PTPN6 Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of PTPN6 Antibody in HL-60 cell line lysates (35 ug/lane)
Western blot analysis of PTPN6 using rabbit polyclonal PTPN6 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN6 gene (Lane 2). (2ug/ml)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTPN6 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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