You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1260868 |
---|---|
Category | Antibodies |
Description | PTGS2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:4000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 80kDa |
Target | PTGS2 |
UniProt ID | P35354 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | COX-2, prostaglandin-endoperoxide synthase 2 (pros Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:4000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Cox2 antibody (orb1260868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human liver cancer using Cox2 antibody (orb1260868) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using Cox2 antibody (orb1260868) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using Cox2 antibody (orb1260868) at dilution of 1:100. Blue: DAPI for nuclear staining.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Porcine, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating