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Catalog Number | orb1938116 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB17409 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 68996 Da |
Target | This PTGS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 363-391 amino acids from the Central region of human PTGS2. |
UniProt ID | P35354 |
NCBI | NP_000954.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Prostaglandin G/H synthase 2, Cyclooxygenase-2, CO Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-PTGS2 Antibody (Center P378) at 1:2000 dilution + HACAT whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of PTGS2 Antibody (Center P378) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
PTGS2 Antibody (Center P378) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of PTGS2 Antibody (Center P378) for immunohistochemistry. Clinical relevance has not been evaluated.
PTGS2 Antibody (Center P378) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the PTGS2 antibody detected the PTGS2 protein (arrow).