PTEN Antibody

• Catalog Number: orb1826252
• Category: Antibodies
• Description: PTEN (phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase) contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. It was identified as a tumor suppressor that is mutated in a large number of cancers, including sporadic brain, breast, kidney, and prostate cancers.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PTEN/7345
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG, kappa
• Immunogen: Recombinant full-length human PTEN protein was used as the immunogen for the PTEN antibody.
• Dilution range: ELISA (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This PTEN antibody is available for research use only.
• UniProt ID: P60484
• Storage: Aliquot the PTEN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate carcinoma tissue with PTEN antibody (clone PTEN/7345) at 2 ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PTEN antibody (clone PTEN/7345) as confirmation of integrity and purity.

IHC staining of FFPE human kidney tissue with PTEN antibody (clone PTEN/7345) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with PTEN antibody (clone PTEN/7345) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.