PSEN1 antibody

• Catalog Number: orb584174
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PSEN1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSEN1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: PSEN1
• UniProt ID: P49768
• Protein Sequence: Synthetic peptide located within the following region: EGDPEAQRRVSKNSKYNAESTERESQDTVAENDDGGFSEEWEAQRDSHLG
• NCBI: NP_000012
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: AD3, FAD, PS1, PS-1, S182, ACNINV3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Amount and Sample Type: 500 ug mouse brain homogenate, Amount of IP Antibody: 6 ug, Primary Antibody: PSEN1, Primary Antibody dilution: 1:500, Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594, Secondary Antibody dilution: 1:5000, Gene Name: PSEN1.

Host: Rabbit, Target Name: PSEN1, Sample Type: Human 721_B, Antibody dilution: 1.0 ug/ml. PSEN1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Host: Rabbit, Target Name: PSEN1, Sample Type: Human Adult Placenta, Antibody dilution: 1.0 ug/ml.

Immunohistochemistry with Small intestine tissue.

Lanes: 1. Mouse WT brain extract (80 ug) 2. Rat brain extract (80 ug), Primary Antibody dilution: 2 ug/ml, Secondary Antibody: IRDye 800CW goat anti-rabbit, Secondary Antibody dilution: 1:20000, Gene Name: PSEN1.

The PSEN1 antibody works fine in 4% paraformaldehyde fixed tissue sections (30 um), they were cut on a sliding, freezing microtome. We stained it overnight 1:500 followed with a biotinylated secondary, followed by an Avidin/peroxidase tertiary antibody, final staining with metal-intensified DAB. Similarly, both LRP antibodies work fine (stain appropriately) with this procedure. We are still evaluating the BACE1 antibody, it seems to work, too.

WB Suggested Anti-PSEN1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Human brain.