Protein Molecular Weight Marker

• Catalog Number: orb1567188
• Category: Proteins
• Description: Protein Molecular Weight Marker
• Tested applications: SDS-PAGE
• Concentration: 1X
• Form/Appearance: Liquid (in 1x Loading Buffer)
• Purity: Protein Molecular Weight Marker is supplied in 1X loading buffer and is ready-to-use. It contains: [62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue.] This product are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C.
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None. 2.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
• Alternative names: Protein ladder, Protein marker, Protein standard,
• Note: For research use only
• Application notes: Protein Molecular Weight Marker has been tested and is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats
• Expiration Date: 6 months from date of receipt.

SDS PAGE of Protein Molecular Weight Marker, 14-120 kDa.

SDS PAGE Results of Protein Molecular Weight Marker 14-120 kDa. Lane 1: Opal Prestained Molecular Weight Marker (11-245 kDa). [7 µL] Lane 2: Protein Molecular Weight Marker (14-120 kDa) (p/n orb348606). [5 µL] 4-20% Gel, Coomassie Stained.

SDS-Page of Protein Molecular Weight Marker. Lane 1: Protein Molecular Weight Marker 12.5 uL. Lane 2: Protein Molecular Weight Marker 10.0 uL. Lane 3: Protein Molecular Weight Marker 7.5 uL. Lane 4: Protein Molecular Weight Marker 5.0 uL.