Protein G antibody (Peroxidase)

• Catalog Number: orb344737
• Category: Antibodies
• Description: Protein G antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA
• Isotype: IgG
• Immunogen: Protein G [Streptococcus species]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti- Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species]. Cross reactivity against Protein G from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Protein G Antibody HRP Conjugation, Pe
• Note: For research use only
• Application notes: Anti-Protein G Peroxidase Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Anti-Protein G Antibody Peroxidase Conjugated. Lane A: Protein G at 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: Anti-Protein G HRP Conjugated at 1 µg/mL for 1 hr at RT. Secondary Antibody: none. Block: BlockOut Buffer (p/n orb348644) for 30 mins. Exposure: 6secs.