Protein G antibody (Peroxidase)

• Catalog Number: orb344736
• Category: Antibodies
• Description: Protein G antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA
• Isotype: IgG
• Immunogen: Protein G [Streptococcus species]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: Anti-Protein G is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species]. Cross reactivity against Protein G from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Protein G Antibody HRP Conjugation, Pe
• Note: For research use only
• Application notes: Anti-Protein G Peroxidase Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot Results of Anti-Protein G Antibody Peroxidase Conjugated. Lane A: Protein G at 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: Anti-Protein G HRP Conjugated at 1 µg/mL for 1 hr at RT. Secondary Antibody: none. Block: BlockOut Buffer (p/n orb348644) for 30 mins. Exposure: 6secs.