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Catalog Number | orb344735 |
---|---|
Category | Antibodies |
Description | Protein G antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA |
Isotype | IgG |
Immunogen | Protein G [Streptococcus species] |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000 |
Form/Appearance | Lyophilized |
Purity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Protein G [Streptococcus species]. Cross reactivity against Protein G from other tissues and species may occur but have not been specifically determined. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | rabbit anti-Protein G Antibody HRP Conjugation, Pe Read more... |
Note | For research use only |
Application notes | Anti-Protein G Peroxidase Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Protein G [Streptococcus species] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
Dot Blot Results of Anti-Protein G Antibody Peroxidase Conjugated. Lane A: Protein G at 100 ng, 33.33 ng, 11.11 ng, 3.70 ng, 1.23 ng. Primary Antibody: Anti-Protein G HRP Conjugated at 1 µg/mL for 1 hr at RT. Secondary Antibody: none. Block: BlockOut Buffer (p/n orb348644) for 30 mins. Exposure: 6secs.
FACS, IF, IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
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