Protein A antibody (Biotin)

• Catalog Number: orb344383
• Category: Antibodies
• Description: Protein A antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Isotype: IgG
• Immunogen: Protein A [Staphylococcus aureus]
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Form/Appearance: Lyophilized
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus]. Cross reactivity against Protein A from other sources is unknown.
• Conjugation: Biotin
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Protein A Antibody biotin conjugated, go
• Note: For research use only
• Application notes: This product has been assayed against 1.0 ug of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Goat anti-Protein A Antibody. Lane 1: Protein A Reduced. Lane 2: Protein A Reduced. Lane 3: Protein A Non-Reduced. Lane 4: Protein A Non-Reduced. Load: Lane 1 and 3 - 1.0 µg per lane, Lane 2 and 4 - 0.25 ug per lane. Primary antibody: Protein A antibody at 1:5000 for overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:10000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa for Protein A. Other band(s): Protein A splice variants and isoforms.