Progesterone Receptor Antibody

• Catalog Number: orb1826046
• Category: Antibodies
• Description: Progesterone is one of the central regulators of female reproduction. In breast development, progesterone is involved in the formation of lobular-alveolar structures and also affects differentiation in the breast by modulation of milk protein synthesis. The cellular effects of progesterone are mediated through progesterone receptors (PR). PR, a protein with 946 amino acids, is a ligand-activated transcription factor member of the steroid receptor super family of nuclear receptors. PR is predominantly expressed in female sex steroid responsive tissues such as the mammary gland, uterus and ovary but is also found in other tissues such as endocrine cells of the Langerhans' islets. The estrogen receptor (ER) and PR status has been used for over 20 years as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PGR/8099R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the Progesterone Receptor antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Progesterone Receptor antibody is available for research use only.
• UniProt ID: P06401
• Storage: Aliquot the Progesterone Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human breast cancer with PR antibody (clone PGR/8099R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PR (PGR/8099R) as confirmation of integrity and purity.