PRMT5

• Catalog Number: orb20273
• Category: Antibodies
• Description: Goat polyclonal to PRMT5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 72.7; 71.3
• Target: PRMT5
• Entrez: 10419
• Protein Sequence: RACREKDRDPEAQ
• RRID: AB_10749410
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PRMT5 antibody, anti protein arginine methylt
• Note: For research use only
• Application notes: This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1).ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 70kDa band observed in Jurkat lysates (calculated MW of 72.7kDa and 71.3kDa according to NP_006100.2; NP_001034708.1). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.