PRMT5 Antibody (C-term)

• Catalog Number: orb27941
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HA Tag is derived from an epitope of the influenza hemagglutinin protein. This has been used extensively as a general epitope tag in expression vectors. This tag facilitates the detection, isolation, and purification of the proteins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0117
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, IHC-P: 1:50-100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 72684
• Target: PRMT5
• UniProt ID: O14744
• NCBI: NP_001034708.1, NP_006100.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Protein methyltransferase JBP1 antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-PRMT5 Pab is used in Western blot to detect PRMT5 in HL60 cell lysate.