PRKCA Antibody / PKC alpha

• Catalog Number: orb699753
• Category: Antibodies
• Description: Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 133
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2a, kappa
• Immunogen: Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This PRKCA antibody is available for research use only.
• UniProt ID: P17252
• Storage: Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the PRKCA antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with PRKCA antibody (clone 133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with PRKCA antibody (clone 133). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.