PRKCA Antibody

• Catalog Number: orb1263537
• Category: Antibodies
• Description: PRKCA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PKC alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-329 amino acids from the C-terminal region of human PKC alpha.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 77 kDa
• Target: PRKCA
• UniProt ID: P17252
• NCBI: P17252
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Protein kinase C alpha type, PKC-A, PKC-alpha, PRK
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of PKC alpha Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-PKCalpha Pab in placenta lysate

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.