PRAS40 (phospho-T246) antibody

• Catalog Number: orb304682
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRAS40 (phospho-T246)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T246 of human PRAS40 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: AKT1S1
• Entrez: 84335, 67605
• UniProt ID: Q96B36, Q9D1F4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PRAS40 antibody, anti Proline-rich AKT1 subst
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PRAS40 (Phospho-T246) expression in HEK293T (A), A549 (B), mouse kidney (C), mouse testis (D), rat kidney (E), rat testis (F) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 40 kD)

Immunohistochemical analysis of PRAS40 (Phospho-T246) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.