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Catalog Number | orb1258283 |
---|---|
Category | Antibodies |
Description | PPP4C Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 36kDa |
Target | PPP4C |
UniProt ID | P60510 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | PPP4C, protein phosphatase 4 (formerly X), catalyt Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PPP4C antibody (orb1258283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat liver using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using PPP4C antibody (orb1258283) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using PPP4C antibody (orb1258283). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug PPP4C antibody (orb1258283). Western blot was performed from the immunoprecipitate using PPP4C antibody (orb1258283) at a dilution of 1:1000.
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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