PPCS Antibody

• Catalog Number: orb1260731
• Category: Antibodies
• Description: PPCS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human PPCS (NP_078940.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 34kDa
• Target: PPCS
• UniProt ID: Q9HAB8
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CMD2C, phosphopantothenate--cysteine ligase, PPC s
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PPCS antibody (orb1260731) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.