POU2AF1 Antibody / BOB-1

• Catalog Number: orb1826019
• Category: Antibodies
• Description: BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BOB1/7468
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: A recombinant partial protein (within amino acids 148-255) from the human protein was used as the immunogen for the POU2AF1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This POU2AF1 antibody is available for research use only.
• UniProt ID: Q16633
• Storage: Aliquot the POU2AF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with BOB1 antibody (clone BOB1/7468). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with BOB1 antibody (clone BOB1/7468). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human placental tissue with BOB1 antibody (clone BOB1/7468) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free BOB1 antibody (clone BOB1/7468) as confirmation of integrity and purity.