Porcine TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit

• Catalog Number: orb1946421
• Category: Assays and Kits
• Description: Porcine TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
• Reactivity: Porcine
• Range: 6.25-400 nmol/ml
• Target: TBARS
• Sensitivity: 3.55 nmol/mL
• Alternative names: TBARS; Thiobarbituric Acid Reactive Substance
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thiobarbituric Acid Reactive Substance(TBARS) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thiobarbituric Acid Reactive Substance(TBARS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of Thiobarbituric Acid Reactive Substance(TBARS) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Expiration Date: Please enquire.