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Catalog Number | orb80409 |
---|---|
Category | Tools |
Description | Recombinant of Porcine IL 1 beta protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP |
Source | Escherichia Coli |
Biological Activity | The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
Storage | Stability: Lyophilized IL 1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL 1 beta should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The IL 1 beta was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose. |
Alternative names | Catabolin, Lymphocyte-activating factor (LAF), End Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 12 months from date of receipt. |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses | |
17.6 kDa |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
17.5 KDa | |
E.Coli |
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