Porcine F1+2 ELISA Kit

• Catalog Number: orb779892
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2(F1+2).
• Tested applications: ELISA
• Reactivity: Porcine
• Range: 6.25-400 ng/mL
• Concentration: 400 ng/mL
• Sensitivity: 2.52 ng/mL
• Protein Length: 3.5h
• Alternative names: F1+2
• Note: For research use only
• Application notes: Standard: 400 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig F1+2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig F1+2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig F1+2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma and other biological fluids
• Expiration Date: Please enquire.