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Catalog Number | orb782501 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androstenedione(ASD) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Androstenedione(ASD). |
Tested applications | ELISA |
Reactivity | Porcine |
Range | 156.25-10000 pg/mL |
Concentration | 10000 pg/mL |
Sensitivity | 48.8 pg/mL |
Protein Length | 2h |
Alternative names | 4-Androstenedione ELISA Kit, 4-Androstene-3,17-Dio Read more... |
Note | For research use only |
Application notes | Standard: 10000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig ASD protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig ASD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig ASD in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
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