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Catalog Number | orb1946478 |
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Category | Assays and Kits |
Description | Porcine ADM(Adrenomedullin) ELISA Kit |
Reactivity | Porcine |
Range | 15.63-1000 pg/mL |
Target | ADM |
Sensitivity | 4.85 pg/mL |
Alternative names | AM; PAMP; Proadrenomedullin N-20 terminal peptide; Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adrenomedullin(ADM) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenomedullin(ADM). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenomedullin(ADM) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | Serum, plasma and other biological fluids. |
Expiration Date | Please enquire. |
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