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Catalog Number | orb1256029 |
---|---|
Category | Antibodies |
Description | POLR1D Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human POLR1D (NP_057056.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 15kDa |
Target | POLR1D |
UniProt ID | Q9Y2S0 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | POLR1D, FLJ20616, MGC9850, POLR1C, RPA16, RPA9, RP Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using POLR1D antibody (orb1256029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.
Immunofluorescence analysis of A549 cells using POLR1D antibody (orb1256029). Blue: DAPI for nuclear staining.
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