Podoplanin Antibody / gp36

• Catalog Number: orb1824548
• Category: Antibodies
• Description: It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rPDPN/6994
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant human Podoplanin protein fragment (within amino acids 1-100) was used as the immunogen for the recombinant PDPN antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant PDPN antibody is available for research use only.
• UniProt ID: Q86YL7
• Storage: Aliquot the recombinant PDPN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with recombinant PDPN antibody (clone rPDPN/6994). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PDPN antibody (clone rPDPN/6994) as confirmation of integrity and purity.