PMEL / gp100 / Melanosome antibody

• Catalog Number: orb2276545
• Category: Antibodies
• Description: PMEL / gp100 / Melanosome antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-617R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P40967
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: 95kDa melanocyte-specific secreted glycoprotein, M
• Note: For research use only
• Application notes: Positive Control: Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin. Negative Control: Colon: PMEL immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Strong PMEL immunostaining in melanocytes of the skin.

Rectum mucosa.

Strong cytoplasmic PMEL immunostaining in all cells of a malignant melanoma of the skin.