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Catalog Number | orb2276543 |
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Category | Antibodies |
Description | PMEL / gp100 / Melanosome antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | Biorbyt-617R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | P40967 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | 95kDa melanocyte-specific secreted glycoprotein, M Read more... |
Note | For research use only |
Application notes | Positive Control: Skin: A moderate to strong cytoplasmic PMEL immunostaining should be seen in all melanocytes of the skin. Negative Control: Colon: PMEL immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Rectum mucosa.
Strong cytoplasmic PMEL immunostaining in all cells of a malignant melanoma of the skin.
Strong PMEL immunostaining in melanocytes of the skin.
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