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Catalog Number | orb548415 |
---|---|
Category | Antibodies |
Description | Plexin A1 Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | AFOO-16 |
Tested applications | FC, ICC, IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human Plexin A1 Plays a role in axon guidance, invasive growth and cell migration. |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
UniProt ID | Q9UIW2 |
Sensitivity | < 10pg/ml |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | WB 1:500-1:2000ICC/IF 1:50-1:200FC 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PLXNA1 expression in HUVEC cell lysate using anti-PLXNA1 antibody (orb548415). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNA1 antigen affinity purified polyclonal antibody (Catalog # orb548415) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for PLXNA1.
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