Plectin Antibody

• Catalog Number: orb259607
• Category: Antibodies
• Description: Plectin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Plectin (2644-2671aa RFIEQEKAKLEQLFQDEVAKAQQLREEQ), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 531791 MW
• UniProt ID: Q15149
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Plectin;PCN;PLTN;Hemidesmosomal protein 1;HD1;Plec
• Note: For research use only
• Application notes: WB: The detection limit for Plectin is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Plectin using anti-Plectin antibody.Lane 1:human HeLa cell.

IF analysis of Plectin using anti-Plectin antibody. Plectin was detected in an immunocytochemical section of U20S cells.

IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of human intestine cancer tissue.

IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of mouse intestine tissue.

IHC analysis of Plectin using anti-Plectin antibody. Plectin was detected in a paraffin-embedded section of rat intestine tissue.