You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb214403 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PLCG1 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Primate, Rat |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1253 of human PLC gamma 1 protein. The exact sequence is proprietary. |
Dilution range | WB: 1-500-1-1000, IF/ICC: 1-100-1-500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | PLCG1 |
Entrez | 18803, 25738, 5335 |
UniProt ID | Q62077, P19174, P10686 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti PLC1 antibody, anti 1-phosphatidylinositol 4, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PLC gamma 1 (Phospho-Y1253) expression in MCF7 EGF-treated (A), A431 (B), mouse brain (C), mouse liver (D), H9C2 EGF-treated (E) whole cell lysates. (Predicted band size: 148 kD; Observed band size: 155 kD)
Immunofluorescent analysis of PLC gamma 1 (Phospho-Y1253) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating