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Catalog Number | orb2276451 |
---|---|
Category | Antibodies |
Description | PLAP antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | Biorbyt-350R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant full-length human ALPP protein |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | P05187 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | Alkaline phosphatase placental type; Alkaline phos Read more... |
Note | For research use only |
Application notes | Positive Control: Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen. Negative Control: Appendix: PLAP immunostaining must be absent in all structures. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
In the placenta, membranous PLAP immunostaining is strong in chorion cells.
Placenta, mature: In the placenta, PLAP immunostaining increases continuously throughout the pregnancy. PLAP immunostaining is strong in the cyto- and syncytiotrophoblast of the mature placenta.
Strong PLAP Immunostaining in all cells of a testicular seminoma.2.
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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