PLAP antibody

• Catalog Number: orb2276450
• Category: Antibodies
• Description: PLAP antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-350R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant full-length human ALPP protein
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P05187
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: Alkaline phosphatase placental type; Alkaline phos
• Note: For research use only
• Application notes: Positive Control: Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen. Negative Control: Appendix: PLAP immunostaining must be absent in all structures. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

In the placenta, membranous PLAP immunostaining is strong in chorion cells.

Placenta, mature: In the placenta, PLAP immunostaining increases continuously throughout the pregnancy. PLAP immunostaining is strong in the cyto- and syncytiotrophoblast of the mature placenta.

Strong PLAP Immunostaining in all cells of a testicular seminoma.2.