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Catalog Number | orb1946554 |
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Category | Assays and Kits |
Description | Plant ABA(Abscisic Acid) ELISA Kit |
Reactivity | Plant |
Range | 1.57-100 ng/mL |
Target | ABA |
Sensitivity | 0.54 ng/mL |
Alternative names | AB-A; abscisin II; Dormin Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Abscisic Acid(ABA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Abscisic Acid(ABA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Abscisic Acid(ABA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | plant tissues |
Expiration Date | Please enquire. |
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