PLAG1 Rabbit Polyclonal Antibody

• Catalog Number: orb1291520
• Category: Antibodies
• Description: PLAG1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PLAG1 (21-120/500aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: PLAG1
• UniProt ID: Q6DJT9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: FGFR1/PLAG1 fusion variant 3; Pleiomorphic adenoma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Heart (Mouse) Cell Lysate at 40 ug, Primary: Anti-PLAG1 (orb1291520) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Sample: Lane 1: Human 293T cell Lysates, Lane 2: Human HUVEC cell Lysates, Lane 3: Human Hela cell Lysates, Lane 4: Human Jurkat cell Lysates, Lane 5: Human U2OS cell Lysates, Primary: Anti-PLAG1 (orb1291520) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kDa, Observed band size: 56kDa.

Sample: Lane 1: Human Huvec cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human Jurkat cell lysates, Lane 4: Human U2OS cell lysates, Primary: Anti-PLAG1 (orb1291520) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 58 kDa.

Tissue/Cell: mouse embryo liver, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PLAG1 Polyclonal Antibody, Unconjugated (orb1291520) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.