Placental Alkaline Phosphatase Antibody / ALPP

• Catalog Number: orb606705
• Category: Antibodies
• Description: PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: ALPP/2899R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant Placental Alkaline Phosphatase antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Placental Alkaline Phosphatase antibody is available for research use only.
• UniProt ID: P05187
• Storage: Store the recombinant Placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Placental Alkaline Phosphatase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human placenta with recombinant Placental Alkaline Phosphatase antibody (clone ALPP/2899R).

SDS-PAGE analysis of purified, BSA-free recombinant Placental Alkaline Phosphatase antibody (clone ALPP/2899R) as confirmation of integrity and purity.

Western blot testing of human JEG-3 cells with recombinant Placental Alkaline Phosphatase antibody (clone ALPP/2899R). Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.