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Catalog Number | orb20577 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PLA2G4A |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 85.2 |
Target | PLA2G4A |
Entrez | 5321 |
Protein Sequence | QEKTFRQQRKEHIR |
RRID | AB_10753888 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti calcium-dependent phospholipid-binding protei Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 85.2kDa according to NP_077734.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20577 (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence analysis of A549 cells using PLA2G4A antibody
Flow cytometric analysis of A549 cells using PLA2G4A antibody
Immunohistochemical staining of Human Uterus using PLA2G4A antibody.
FC, IF, IHC-P, WB | |
Gallus, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Mouse, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Equine, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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