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Catalog Number | orb1262657 |
---|---|
Category | Antibodies |
Description | PLA2G4A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Gallus, Mouse, Rabbit, Rat, Zebrafish |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This PLA2G4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the Central region of human PLA2G4A. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 85 kDa |
Target | PLA2G4A |
UniProt ID | P47712 |
NCBI | P47712 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Cytosolic phospholipase A2, cPLA2, Phospholipase A Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysate from 293 cell line, using PLA2G4A Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human lung carcinoma with PLA2G4A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of PLA2G4A Antibody with NCI-H460 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H292 cells using PLA2G4A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ELISA, FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Mouse, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
FC, IF, IHC-P, WB | |
Equine, Mouse, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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