PKR (PRKR) Antibody (C-term)

• Catalog Number: orb1939463
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB5284
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 62094 Da
• Target: This PKR (PRKR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-550 amino acids from the C-terminal region of human PKR (PRKR).
• UniProt ID: P19525
• NCBI: NP_002750.1, NP_001129123.1, NP_001129124.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interferon-induced, double-stranded RNA-activated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-PRKR Pab is used in Western blot to detect PRKR in mouse uterus tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).

Western blot analysis of EIF2AK2 (arrow) using PRKR Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF2AK2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.